Capture the ion mobility mass spectrometry (TIMS)

Ion mobility spectrometry (Ion mobility spectrometry, IMS) is a powerful analytical technique, has been widely used in the past fifty years, is mainly used in chemical physics and chemistry analysis application.Until recently explored the IMS and MS (IMS - MS) the potential of combination, used for the separation of peptides and proteins, identification and quantitative.

Capture the ion mobility spectrometry (TIMS) is a kind of IMS technology, under the impetus of the ions in the air flow through the TIMS channel funnel (ions).In addition to the air force, ionAt the same time by electric field of the reverse reaction.Under the function of two kinds of force balance, arrested ions can be set in the funnel ions.Weakened by adjusting the electric field intensity, electric field force, can be selectively make different ion successively by ion mobility properties of funnel, so as to achieve the function of the separation.

By integrating TIMS analyzer to quadrupole time-of-flight mass spectrometer (QTOF) front-end, ion can before release for MS analysis for accumulation within a certain time.Polypeptide ion separation and release in TIMS mobility (~ 100 ms), and then tested in the detector, produce TIMS ms heat map;The TIMS separation of peptides into the quadrupole ion in isolation and select ion, the parent ion selective process is in the process of ion release.The last mother ion and fragment ions according to the mobility values for alignment.

TIMS
Capture ion mobility spectrometry (TIMS) is the IMS separation technology in the gas phase, in addition to the HPLC separation and mass spectrometry analysis, to deal with it by increasing the separation dimensions complex samples, improves the peak capacity and compound representation of information.Equally important, TIMS analyzer is also used to accumulation and enrichment of the given values of quality and mobility of ions, thus get unique on sensitivity and speed increase, at the same time increase the separation of dimension.

Using double TIMS technique, now can make of high sensitivity, high speed which proteomics utilization rate of nearly 100% of the ions.Novel design allows ions accumulated in the front, and then according to the ion mobility of ions released in sequence.This process is called PASEF ®(Parallel Accumulation Serial Fragmentation,

Synchronous accumulation continuous fracture) technique.

PASEF ®
In terms of speed, brooke experts redesigned the MS/MS technology, in order to meet the requirements of which proteomics.With the help of PASEF ® technology, can realize > 100 Hz sequencing speed;Low abundance of peptides by multiple choice and rupture, the MS/MS spectra quality significantly improved.

Brooke timsTOF solution can accelerate a variety of research and application, to provide identification and quantitative challenging compounds of confidence and clarity.